Spectroflourometric and spectrophotometric methods for the determination of sitagliptin in binary mixture with metformin and ternary mixture with metformin and sitagliptin degradation product
Bassam M. Ayoub
Department Of pharmaceutical Chemistry
Faculty Of Pharmacy, Cairo University Egypt
Abstract:
Simple, accurate and precise spectroflourometric and spectrophotometric methods have been developed and validated for the determination of sitagliptin (STG) and metformin (MET). Zero order, first derivative, ratio derivative spectrophotometric methods and flourometric methods have been developed. The zero order spectrophotometric method was used for the determination of STG in the range of 50-300 μgmL−1. The first derivative spectrophotometric method was used for the determination of MET in the range of 2-12 μgmL−1 and STG in the range of 50-300 μgmL−1 by measuring the peak amplitude at 246.5 nm and 275 nm, respectively. The first derivative of ratio spectra spectrophotometric method used the peak amplitudes at 232 nm and 239 nm for the determination of MET in the range of 2-12 μgmL−1. The flourometric method was used for the determination of STG in the range of 0.25-110 μgmL−1. The proposed methods used to determine each drug in binary mixture with metformin and ternary mixture with metformin and sitagliptin degradation product that is obtained after alkaline hydrolysis of sitagliptin. The results were statistically compared using one-way analysis of variance (ANOVA). The methods developed were satisfactorily applied to the analysis of the pharmaceutical formulations and proved to be specific and accurate for the quality control of the cited drugs in pharmaceutical dosage forms.
